Action OncoKitDx (Automatic)

Protocol automated using Magnis NGS Prep System

Analysis of the main clinically useful genomic biomarkers associated with the most common solid tumors, using a fully automated protocol and NGS sequencing

24 rxn

NGS sequencing

IMG-365

Illumina platforms

Protocol automated using Magnis NGS Prep System
  • NGS panel for the study of the main clinically relevant biomarkers for the most common solid tumors in adults.
  • It allows detecting SNVs, indels, genetic fusions, CNVs, pharmacogenetic variants, and microsatellite instability analysis (MSI) from a single DNA sample.
  • Bioinformatics analysis with the Datagenomics software and semiautomated generation of reports, including functional and clinical classification of the identified variants.
  • STIDs can be included: Integrated sample identification and tracking system.
  • Based on high sensitivity RNA-probe technology with UMI barcoding (unique molecular identifiers).
  • Mean coverage: 2500X and mean coverage after UMI analysis: 1600X.
  • Coverage (at depth >100x), specificity, sensitivity, repeatability, and reproducibility of this test are over 99%. The uniformity of bases covered at >20x is 98.8%.
  • Technical support starting with the implementation of the first protocol, available both online and on-site. All protocols will be set up in collaboration with your laboratory personnel.
  • STID: Clinical support and genetic counseling. Our team of specialists can help you with any query you may have regarding cancer genetics.
  • Possibility of sending 10% of your samples to our laboratory at no additional cost during periods of excessive laboratory workload or urgent deadlines.
  • Possibility of handling your projects as service at Health in Code laboratories (TAT: 10 working days).
  • It complies with the quality requirements specified by ISO 13485 and ISO 14001 in relation to the materials used in its manufacturing process.

Action OncoKitDx uses a technology for library preparation by mechanical fragmentation and enrichment of regions of interest by hybridization with capture probes. This technology is fully automated. Subsequently, massive sequencing is carried out on Illumina platforms.

Compatible sequencing platforms Illumina NextSeq50, Illumina HiSeq, Illumina NovaSeq
Number of reactions: 24
Number of samples per run: 8 to 12 samples in a cartridge MID 150 cycles on NextSeq
Sequencing: Paired-end (2 x 75 cycles)
Sample type: DNA from peripheral blood and fresh, frozen, and paraffin-embedded tissue
Amount of input DNA 50-200 ng
Limit of detection SNVs, indels, and structural variants: 5%; CNVs: 3 copies for duplications and 1 copy for deletions in samples with non-tumoral cell infiltration over 30%
Fully automated panel for Magnis NGS Prep System Dx equipment
CE-IVD-marked kit and analysis software
target genes SNVs e INDELs: AKT1*, ALK, ARID1A, ATM, ATRX, BAP1, BRAF, BRCA1, BRCA2, CDH1, CHEK2, CTNNB1, EGFR, ERBB2, ESR1, FGFR1, FGFR4, GNA11, GNAQ, H3F3A, HIST1H3B, HIST1H3H, HRAS, IDH1, IDH2, KIT, KRAS, MAP2K1, MET, MLH1, MSH2, MSH6, MTOR, MYC, NRAS, NTRK1, NTRK2, NTRK3, PALB2, PBRM1, PDGFRA, PIK3CA, PMS2 + 5’UTR, PTEN, POLD1, POLE, RET, ROS1, SDHA, SDHB, SDHD, TERT + 5’UTR, TSC1*, TSC2*, TP53 y VHL.

* Secuenciación de hotspot

SVs: ALK (intrón 19), ATP1B1 (intrones 3 y 4), BRAF (intrones 7, 8, 9 y 10), EGFR (intrones 7, 23, 24 y 25), ETV6 (intrones 4 y 5), FGFR2 (intrón 17 y región 3’UTR), FGFR3 (intrón 17 y región 3’UTR), NTRK1 (intrones 8, 9, 10, 11 y 12), NTRK2 (intrones 12 y 15), RET (intrones 9, 10 y 11) y ROS1 (intrones 31, 32, 33, 34 y 35).

DNA from peripheral blood, fresh, frozen and paraffin-embedded tissue

Action OncoKitDx (Automatic)

Fill in the form to request this kit.

Call us at +34 963 212 340

Or write to us at info@healthincode.com