Action PharmaKitDx

Protocol automated using Magnis NGS Prep System

Analysis of the main molecular markers related to the response to drugs, described in pharmacogenomic clinical practice guidelines and technical data sheets of drug regulatory agencies, by means of NGS sequencing.

16 rxn

NGS

IMG-401

Protocol automated using Magnis NGS Prep System
  • Dynamic kit that updates the target genes of the panel according to scientific evidence..
  • Detection of SNVs and INDELs.
  • Detection of CNVs in CYP2D6.
  • Analysis software included.
  • STID: Integrated sample identification system for traceability.
  • Average coverage: > 900, with 99.62% of the bases covered at a depth of 30X.
  • Sensitivity: > 99%.
  • Specificity: > 99%.
  • Repeatability: > 99%.
  • Reproducibility: >99%.
  • It complies with the quality requirements specified by ISO 13485 and ISO 14001 in relation to the materials used in its manufacturing process.
  • Can use STIDs: sample tracking identification numbers for effective sample management
  • Fully-automated panel with a library ready in 9 hours on the Magnis Dx NGS Prep System platform
  • For use in research.

Action PharmaKitDx employs a fully automated technology for library preparation and selection of regions of interest by hybridization with capture probes. Subsequently, massive sequencing is carried out on Illumina platforms.

  • Compatible sequencers: Illumina iSeq, Illumina MiSeq, Illumina NextSeq500, Illumina NextSeq2000, Illumina HiSeq, Illumina NovaSeq
  • Number of reactions: 16.
  • Number of samples per run: Illumina MiSeq V2 (300 cycles): 8
  • Tipo de muestra: ADN procedente de sangre periférica.
  • DNA quantity per sample: 100 ng.
  • Genomic region analyzed: 0.408 Mb.
  • Target genes: ABCB1, ABCB11, ABCB4, ABCB7, ABCC1, ABCC2, ABCC3, ABCC4, ABCC5, ABCC6, ABCC8, ABCC9, ABCG1, ABCG2, ACE, ACKR1, ACYP2, ADA, ADD1, ADGRB3, ADGRL2, ADH1A, ADH1B, ADH1C, ADORA2A, ADRB1, ADRB2, AFF3, AHR, AKR1E2, ALDH1A1, ALDH2, ALOX5, ALPL, AMPD1, ANKK1, APOA5, APOE, ARL14, ATIC, ATP7A, BDNF, BMP2, BMP7, BRINP1, BTG1, C11orf65, C1orf167, C8orf34, CACNA1S, CADM2, CALU, CARD8, CBR3, CCDC179, CCHCR1, CCR5, CD2, CD226, CD69, CD80, CD84, CD86, CDA, CEP72, CES1, CES2, CETP, CHRNA3, CHRNB2, CHST7, CHUK, CLEC2D, CLSTN2, CNTN5, COL22A1, COMT, COQ2, CREB1, CRHR1, CRHR2, CRP, CSMD1, CSMD3, CUL1, CYP17A1, CYP19A1, CYP1A1, CYP1A2, CYP1B1, CYP2A13, CYP2A6, CYP2B6, CYP2C18, CYP2C19, CYP2C8, CYP2C9, CYP2D6, CYP2E1, CYP2F1, CYP2J2, CYP2S1, CYP3A4, CYP3A43, CYP3A5, CYP3A7, CYP4B1, CYP4F2, DCK, DHFR, DHODH, DLG2, DOCK1, DPP6, DPYD, DRD1, DRD2, DRD3, DRD4, DYNC2H1, EGF, EGLN3, EMCN, ENOX1, EPHX1, ERCC1, ESR1, ESR2, ETV1, EYA4, F5, FAAH, FAR1, FCGR2A, FCGR2B, FCGR3A, FDPS, FKBP5, FLT3, FMO2, FMO3, FOXP3, FPGS, FTO, G6PD, GABBR2, GALNT18, GATM, GFRA1, GFRA2, GGCX, GGH, GNB3, GP1BA, GRIK4, GSTM1, GSTP1, GSTT1, HAS3, HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-E, HLA-G, HMGCR, HTR1A, HTR2A, HTR2C, HTR3A, IFNG, IFNGR2, IFNL3, IFNL4, IGHM, IKBKB, IL10, IL12B, IL17A, IL17F, IL18, IL1B, IL1R1, IL2, IL4R, IL6, IL6R, IRAK3, ITGB7, ITPA, KCNE1, KCNH2, KCNIP1, KCNJ11, KIAA0391, KIF6, KLRC1, KLRD1, LMO3, LMX1A, LPA, LRPAP1, LRRC55, LTC4S, MAP2K6, MAP3K1, MAP3K14, MAP3K7, MAPK14, MAPKAPK2, MAPT, MC4R, MED15, MICA, MMP20, MRPL36, MS4A1, MTHFD1, MTHFR, MTR, MTRR, MYD88, NAT1, NAT2, NAV2, NEBL, NEDD4L, NFIB, NFKBIA, NFKBIB, NFKBIE, NLRP3, NQO1, NR1I2, NR2F2, NR3C1, NT5C2, NTRK2, NUBPL, NUDT15, OPRD1, OPRM1, OR2B11, OSR1, P2RY1, P2RY12, PDZD2, PLA2G4A, PNPLA3, PRELID3B, PRKCA, PRSS53, PTGFR, PTGIS, PTGS1, PTGS2, PTPRC, PTPRM, QKI, RAD51B, RHOBTB1, RPS6KA5, RYR1, SCN1A, SCN5A, SCNN1A, SEMA3C, SHMT1, SLC15A1, SLC15A2, SLC16A7, SLC19A1, SLC22A1, SLC22A11, SLC22A2, SLC22A6, SLC28A3, SLC46A1, SLC6A4, SLC9A7, SLCO1A2, SLCO1B1, SLCO1B3, SLCO1C1, SLCO2B1, SOD2, SP3, SRY, STAT4, STRBP, SULT1A1, SULT1A2, SYNM, TANC1, TBXAS1, TCF7L2, TEC, TGFB1, TGFBR2, TLR1, TLR10, TLR2, TLR4, TLR5, TNF, TNFAIP3, TNFRSF1A, TNFRSF1B, TNFSF13B, TP53, TPMT, TRAF1, TUBB2A, TYMS, UGT1A1, UGT1A10, UGT1A3, UGT1A4, UGT1A5, UGT1A6, UGT1A7, UGT1A8, UGT1A9, UGT2B10, UGT2B15, UGT2B17, UGT2B7, UMPS, VDR, VKORC1, WDR27, XPC, XRCC1, YEATS4, ZNF595.

Action PharmaKitDx

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