cKIT dPCR OncoKit

Detection and quantification of the pathogenic variant p.D816V, located in the second tyrosine kinase domain of the KIT gene and associated with systemic mastocytosis (MS), in genomic DNA

24 rxn (dehydrated/freeze-dried)

Digital PCR (dPCR)

IMG-407

QuantStudioTM Absolute QTM Digital PCR System (ThermoFisher Scientific)

  • Quantitative multiplex assay: quantification of the p.D816V variant (NM_000222.3:c.2447A>T; p.Asp816Val) in genomic DNA from peripheral blood.
  • cKIT dPCR OncoKit is intended for use in research and is aimed exclusively at healthcare workers and professionals working in molecular biology.

cKIT dPCR OncoKit is designed to detect and quantify the pathogenic variant p.D816V in the second tyrosine kinase domain of the KIT gene. The kit utilizes digital PCR (dPCR), a highly sensitive, precise, and accurate end-point PCR method that allows absolute quantification of a sample that is subdivided into 20,000 partitions.
The kit employs a combination of oligonucleotides and fluorescent hydrolysis probes in a multiplex assay for relative quantification of the mutated allele against the wild-type allele.

  • Sample type: Genomic DNA from peripheral blood
  • Positive control: Synthetic DNA with the pathogenic variant p.D816V in heterozygosity
  • Necessary amount of DNA: 65 ng.
  • Limit of detection: 0,06% ± 1,8E-04
  • Limit of quantification: 0,1%
  • Number of reactions per sample: 1
  • Number of targets: 2 (wild-type and altered alelle)
  • Duration of the dPCR program: 1 h 45 min.

cKIT dPCR OncoKit

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