Coeliac ImmuneKit

Detection of the most common alleles associated with higher susceptibility to coeliac disease, including risk alleles encoding proteins DQ2.5, DQ2.2, DQ8, and DQ7.5:
· DQ2.5: HLA-DQA1*05 / HLA-DQB1*02
· DQ2.2: HLA-DQA1*02 / HLA-DQB1*02
· DQ8: HLA-DQA1*03 / HLA-DQB1*03:02
· DQ7.5: HLA-DQA1*05 / HLA-DQB1*03:01
· Allele load analysis for allele HLA-DQB1*02

48 x 4 rxn

Real-time PCR


  • Multiplexed assay: can detect the presence of alleles associated with coeliac disease while minimizing the number of reactions.
  • High sensitivity and specificity: >90%
  • Allows determining allele load for the risk allele HLA-DQB1*02 by discriminating between the risk allele and the remaining alleles of the HLA-DQB1 gene (DQB1*03, DQB1*04, DQB1*05, and DQB1*06).
  • Includes an endogenous human control to confirm sample quality and integrity.
  • Includes a positive control that allows amplifying all targets of the kit, guaranteeing the functionality of PCR systems and aiding interpretation of quantitative analysis.
  • Compliant with the recommendations of the Spanish Coeliac Disease Society (Sociedad Española de Enfermedad Celíaca, SEEC).
  • This kit is valid for use in research.

Coeliac ImmuneKitMultiplexed real-time PCR uses a sequence-specific oligonucleotide and hydrolysis probe system that allows for the specific discrimination of targets of interest.

  • Necessary amount of DN: 200 ng in total
  • Sample type: genomic DNA (peripheral blood)
  • Number of reactions per sample: 4
  • Number of targets: 8
  • Set-up and preparation: 30 min.
  • Duration of the PCR program: 1h 40 min.
  • Compatible thermal cyclers: 7500 FAST Real-Time PCR System (Applied Biosystems) StepOne Plus Real-Time PCR System (Applied Biosystems)

Coeliac ImmuneKit

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