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Coeliac ImmuneKit

Description

Detection of the most common alleles associated with higher susceptibility to coeliac disease, including risk alleles encoding proteins DQ2.5, DQ2.2, DQ8, and DQ7.5:
· DQ2.5: HLA-DQA1*05 / HLA-DQB1*02
· DQ2.2: HLA-DQA1*02 / HLA-DQB1*02
· DQ8: HLA-DQA1*03 / HLA-DQB1*03:02
· DQ7.5: HLA-DQA1*05 / HLA-DQB1*03:01
· Allele load analysis for allele HLA-DQB1*02

Format

48 x 4 rxn

Technology

Real-time PCR

Reference

IMG-420

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Characteristics
Technology
Technical specifications
Documentation

Multiplexed assay: can detect the presence of alleles associated with coeliac disease while minimizing the number of reactions.
High sensitivity and specificity: >90%
Allows determining allele load for the risk allele HLA-DQB1*02 by discriminating between the risk allele and the remaining alleles of the HLA-DQB1 gene (DQB1*03, DQB1*04, DQB1*05, and DQB1*06).
Includes an endogenous human control to confirm sample quality and integrity.
Includes a positive control that allows amplifying all targets of the kit, guaranteeing the functionality of PCR systems and aiding interpretation of quantitative analysis.
Compliant with the recommendations of the Spanish Coeliac Disease Society (Sociedad Española de Enfermedad Celíaca, SEEC).
Compliant with quality specifications by the UNE-EN ISO 9001 Quality Management System standard regarding manufacturing materials.
This kit is valid for use in research.

Coeliac ImmuneKitMultiplexed real-time PCR uses a sequence-specific oligonucleotide and hydrolysis probe system that allows for the specific discrimination of targets of interest.

Necessary amount of DN: 200 ng in total
Sample type: genomic DNA (peripheral blood)
Number of reactions per sample: 4
Number of targets: 8
Set-up and preparation: 30 min.
Duration of the PCR program: 1h 40 min.
Compatible thermal cyclers: 7500 FAST Real-Time PCR System (Applied Biosystems) StepOne Plus Real-Time PCR System (Applied Biosystems)

Coeliac ImmuneKit: Instructions for use

Coeliac ImmuneKit: MSDS (english)

Analysis certificate