Hereditary OncoKitDx

NGS sequencing of 50 genes associated with hereditary cancer predisposition

48 rxn (Dilution)

NGS

IMG-316

Illumina platforms

  • Dynamic kit that updates the target genes of the panel according to scientific evidence.
  • Type of mutations analyzed: SNVs, INDELs, CNVs, ALUs and other large insertions.
  • Coverage: 99.5% of bases covered at a depth of 50X.
  • Uniformity: 99.2% of the bases covered at >20% of the average coverage and 90.0% of the bases covered at >50% of the average coverage.
  • Sensitivity: > 99.9%
  • Specificity: > 99.9%
  • It complies with the quality requirements specified by ISO 13485 and ISO 14001 in relation to the materials used in its manufacturing process.
  • For in vitro diagnostic use.

Hereditary OncoKitDx employs library preparation technology by enzymatic fragmentation and enrichment of regions of interest by hybridization with capture probes. Subsequently, massive sequencing is carried out on Illumina platforms.

Compatible sequencers Illumina iSeq, Illumina MiSeq, Illumina NextSeq500, Illumina NextSeq2000, Illumina HiSeq, Illumina NovaSeq
Number of reactions 48
Number of samples per run Illumina Miseq Micro (300 ciclos): 12

Illumina Miseq V2 (300 ciclos): 48

Sequencing: Paired-end (2 x 150)
Amount of DNA per sample 20-26 ng
Size of regions of interest 184 Kb
Target genes 50 genes: APC, ATM, BARD1, BMPR1A, BRCA1, BRCA2, BRIP1, CDH1, CDK4, CDKN2A, CHEK2, EPCAM, FAM175A(ABRAXAS1), FH, KIF1B, MAX, MEN1, MET, MLH1, MLH3, MRE11, MSH2, MSH3, MSH6, MUTYH, NBN, NF1, NTHL1, PALB2, PIK3CA, PMS2, POLD1, POLE, PTEN, RAD50, RAD51C, RAD51D, RB1, RET, SDHA, SDHAF2, SDHB, SDHC, SDHD, SMAD4, STK11, TMEM127, TP53, VHL, XRCC2
Type of sample Blood or Saliva

Hereditary OncoKitDx

Fill in the form to request this kit.

Call us at +34 963 212 340

Or write to us at info@healthincode.com