Home / Analysis kits / Imegen-Alpha-1-AT

Imegen-Alpha-1-AT

Description

Detection of p.Glu342Lys (PI-Z) and p.Glu264Val (PI-S) changes in the SERPINA gene, mainly associated with lung diseases

Format

48 x 2 rxn

Technology

real-time PCR

Reference

IMG-211

Compatible equipment

7500 FAST Real-Time PCR System (ThermoFisher), StepOnePlus Real-Time PCR System (ThermoFisher), StepOne Real-Time PCR System (Thermo Fisher), LightCycler 480 (Roche), LightCycler Capillar (Roche)

Kit Health in Code
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  • Assay that determines the presence of mutations g.Glu342Lys (PI-Z; rs28929474; c.1096G>A) and g.Glu264Val (PI-S; rs17580; c.863A>T) and the normal allele of the SERPINA1 (1)in a single PCR reaction.
  • It includes a positive control that simulates a heterozygous genotype for each mutation, which guarantees the functionality of the PCR system and helps interpretation in qualitative analysis.
  • For in vitro diagnostic use.

Imegen-Alfa-1-AT Real-time PCR uses a system of sequence-specific oligonucleotides and fluorescent hydrolysis probes that allow specific targets of interest to be discriminated.

  • Amount of DNA needed: 100 ng total
  • Sample type: Peripheral blood (Germinal)
  • Number of reactions per sample: 2
  • Number of targets: 4
  • Manual working time: 30 min
  • Duration of the PCR program: 1h 15min
  • Compatible cyclers: Real-time PCR cycler with FAMTM and VIC® channels
Imegen-Alpha-1-AT: Instructions for use
Imegen-Alpha-1-AT: MSDS
Analysis certificate

Imegen-Alpha-1-AT

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