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Imegen-Coeliac

Description

Detection of the most frequent alleles associated with a greater susceptibility to developing celiac disease (HLA-DQA1*05, HLA-DQA1*03, HLA-DQB1*03:02 and HLA-DQB1*02)

Format

48 x 3 rxn

Technology

Real-time PCR

Reference

IMG-341
Also available IMG-341 RUO

Compatible equipment

7500 FAST Real-Time PCR System (ThermoFisher), StepOne Plus Real-Time PCR System (Thermo Fisher)

Kit Health in Code
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  • Multiplexed assay: allows the presence of risk alleles associated with celiac disease to be determined, minimizing the number of reactions.
  • High sensitivity and specificity: >90%
  • It allows determining the zygosity of the HLA-DQB1:02 risk allele, discriminating between the risk allele and the rest of the HLA-DQB1 alleles ( DQB1*03 , DQB1*04 , DQB1*05 and DQB1*06 )
  • It includes a positive control that allows to amplify all the targets of the kit, guaranteeing the functionality of the PCR systems and helping the interpretation in the qualitative analysis.
  • Includes an endogenous control to confirm sample quality and integrity.
  • It conforms to the recommendations of the Spanish Society of Celiac Disease (SEEC).
  • For in vitro diagnostic use.

Imegen-Coeliac Real-time multiplexed PCR uses a system of sequence-specific oligonucleotides and hydrolysis probes that allow specific targets of interest to be discriminated.

  • Amount of DNA needed: 150 ng total
  • Sample type: Peripheral blood (Germinal)
  • Number of reactions per sample: 3
  • Number of targets: 6
  • Manual working time: 30 min
  • Duration of the PCR program: 1h 40 min
  • Compatible cyclers: real-time PCR cycler with FAMTM and VIC® channels
Imegen-Coeliac: Instructions for use CE/IVD
Imegen-Coeliac: MSDS (english)
Imegen-Coeliac:
Analysis certificate

Related kits

Imegen-HLA-B27

IMG-289

Imegen-HLA-B57:01

IMG-306

Imegen-Coeliac

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