Imegen-Coeliac

Detection of the most frequent alleles associated with a greater susceptibility to developing celiac disease (HLA-DQA1*05, HLA-DQA1*03, HLA-DQB1*03:02 and HLA-DQB1*02)

48 x 3 rxn

Real-time PCR

IMG-341

7500 FAST Real-Time PCR System (ThermoFisher), StepOne Plus Real-Time PCR System (Thermo Fisher)

  • Multiplexed assay: allows the presence of risk alleles associated with celiac disease to be determined, minimizing the number of reactions.
  • High sensitivity and specificity: Discriminates in 100% of the samples the alleles and subtypes associated with an increased risk of developing celiac disease.
  • It allows determining the zygosity of the HLA-DQB1:02 risk allele, discriminating between the risk allele and the rest of the HLA-DQB1 alleles ( DQB1*03 , DQB1*04 , DQB1*05 and DQB1*06 )
  • It includes a positive control that allows to amplify all the targets of the kit, guaranteeing the functionality of the PCR systems and helping the interpretation in the qualitative analysis.
  • Includes an endogenous control to confirm sample quality and integrity.
  • It conforms to the recommendations of the Spanish Society of Celiac Disease (SEEC).
  • It complies with the quality requirements specified by ISO 13485 and ISO 14001 in relation to the materials used in its manufacturing process.
  • For in vitro diagnostic use.

Imegen-Coeliac Real-time multiplexed PCR uses a system of sequence-specific oligonucleotides and hydrolysis probes that allow specific targets of interest to be discriminated.

  • Amount of DNA needed: 150 ng total
  • Sample type: Peripheral blood (Germinal)
  • Number of reactions per sample: 3
  • Number of targets: 6
  • Manual working time: 30 min
  • Duration of the PCR program: 1h 40 min
  • Compatible cyclers: real-time PCR cycler with FAMTM and VIC® channels

Imegen-Coeliac

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