Home / Analysis kits / Imegen-DM1

Imegen-DM1

Description

Detection of CTG expansion in the DMPK gene associated with Myotonic Dystrophy Type 1 (DM1)

Format

12 rxn

Technology

Fragment Analysis

Reference

IMG-173

Compatible equipment

3730xl DNA Analyzer (ThermoFisher Scientific)

Kit Health in Code
Request Kit
  • Semi-quantitative analysis that generally allows determining the number of repetitions of the CTG expansion of the DMPK gene by means of PCR.
  • Amplification range of 100-500 bp.
  • Includes TP-PCR systems to detect described expansions leading to amplicon sizes >500 bp.
  • Includes a positive control for normal alleles of 5, 12, and 36 replicates.
  • Expansion detection capacity around 150 repetitions.
  • It complies with the quality requirements specified by ISO 13485 and ISO 14001 in relation to the materials used in its manufacturing process.
  • For in vitro diagnostic use.

Imegen-DM1 allows the analysis of CTG triplet expansions in the DMPK gene associated with Myotonic Dystrophy Type 1 (DM1) by means of conventional PCR and capillary electrophoresis. In addition, the kit offers a TP-PCR (triple repeat primed PCR) system to detect expansions in DM1 that are not detectable with conventional PCR.

  • Amount of DNA needed: 500 ng total
  • Sample type: Peripheral blood (Germinal)
  • Number of reactions per sample: 2 (1 PCR and 1 TP-PCR)
  • Number of targets: 1
  • Manual work time: 45 minutes
  • Duration of the PCR/TP-PCR program: 2 h 15 min
  • Compatible thermal cyclers: Any conventional thermal cycler
  • Sequencing with polymers compatible with the label 6-Carboxyfluorescein (6-FAM)
Imegen-DM1: Instructions for use
Imegen-DM1: MSDS
Analysis certificate

Imegen-DM1

Fill in the form to request this kit.

Call us at +34 963 212 340

Or write to us at info@healthincode.com

"*" indicates required fields

Consentimiento*
This field is for validation purposes and should be left unchanged.