Imegen-Factor II dPCR dry

Genotyping of the mutation c.*97G>A (20210G>A) of the F2 gene (Prothrombin) associated with Hereditary Thrombophilia

48 rxn

Digital PCR


QuantStudio 3D (ThermoFisher)

  • Qualitative assay that allows determining the presence of the pathogenic allele c.*97G>A (20210G> A) (NM_000506.4) and the normal allele of the F2 gene in a single multiplexed PCR reaction.
  • The target to detect the presence of the native genotype (20210G) is included as a control of DNA quality and integrity.
  • The kit includes a positive control heterozygous for the G to A transition at position 20210 of the prothrombin gene (20210G>A).
  • The detection limit for genotyping is set at 10 ng of genomic DNA.
  • For research use only, aimed at professionals in the molecular biology industry.

Imegen-Factor II dPCR dryDigital PCR or dPCR is a highly precise technique that uses a combination of oligonucleotides and fluorescent hydrolysis probes in an assay aimed at simultaneously amplifying and specifically detecting the targets of interest.

  • Amount of DNA: 60 ng
  • Sample type: Peripheral blood
  • Number of reactions per sample: 1
  • Number of targets: 2
  • Manual working time: 30 min
  • Duration of the dPCR program: 1h 20 min – 2h
  • Supported Platforms: QuantStudio 3D Digital PCR System (ThermoFisher Scientific) and QX200™ Droplet Digital™ PCR System (Bio Rad)

Imegen-Factor II dPCR dry

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