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Imegen-Factor V dPCR dry

Description

Genotyping of the c.1601G>A mutation; p.Arg534Gln (R506Q) of the F5 gene (Leiden) associated with Hereditary Thrombophilia

Format

48 rxn

Technology

Digital PCR

Reference

IMG-332

Compatible equipment

QuantStudio 3D (ThermoFisher)

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  • Qualitative assay that allows simultaneous detection of the native F5 genotype (c.1601G) and the particular mutation in the F5 gene (NM_000130.4: c.1601G>A; p. Arg534Gln) in a single multiplexed PCR reaction.
  • The target to detect the presence of the native genotype (c.1601G) is included as a control of DNA quality and integrity.
  • The kit includes a positive control heterozygous for the G to A transition at position c.1601 of the Leiden gene (c.1601G>A; p.Arg534Gln).
  • The detection limit for genotyping is set at 10 ng of genomic DNA.
  • For research use only, aimed at professionals in the molecular biology industry.

Imegen-Factor V dPCR dryDigital PCR or dPCR is a highly precise technique that uses a combination of oligonucleotides and fluorescent hydrolysis probes in an assay aimed at simultaneously amplifying and specifically detecting the targets of interest.

  • Amount of DNA needed: 60 ng of DNA per reaction
  • Sample type: Peripheral blood
  • Number of reactions per sample: 1
  • Number of targets: 2
  • Manual working time: 30 min
  • Duration of the dPCR program: 1h 20 min – 2h
  • Supported Platforms: QuantStudio 3D Digital PCR System (ThermoFisher Scientific) and QX200™ Droplet Digital™ PCR System (Bio Rad)
Imegen-Factor V dPCR dry: Instructions for use
Imegen-Factor V dPCR dry: MSDS
Analysis certificate

Related kits

Imegen-Factor II dPCR dry

IMG-331

Imegen-Factor V dPCR dry

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