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Imegen-Friedreich

Description

Detection of GAA expansion in the FXN gene

Format

12 rxn

Technology

Fragment Analysis

Reference

IMG-155

Compatible equipment

3730xl DNA Analyzer (ThermoFisher Scientific)

Kit Health in Code
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  • Semi-quantitative analysis of the number of repetitions of the GAA expansion of the FXN gene by PCR.
  • Amplification range of 100-500 bp.
  • Includes TP-PCR systems to detect described expansions leading to amplicon sizes >500 bp.
  • For research use only, intended for professionals in the field of molecular biology.

Imegen-Friedreich allows analysis of GAA triplet expansions in the FXN gene by conventional PCR and capillary electrophoresis. In addition, the kit offers a TP-PCR (triple repeat primed PCR) system to detect expansions not detectable with conventional PCR.

  • Amount of DNA needed: 50 ng total
  • Sample type: peripheral blood (Germinal)
  • Number of reactions per sample: 2
  • Number of targets: 1
  • Manual work time: 45 minutes
  • Duration of the PCR/TP-PCR program: 2 h 15 min
  • Compatible thermal cyclers: any conventional thermal cycler
  • Sequencing with polymers compatible with the label 6-Carboxyfluorescein (6-FAM)
Imegen-Friedreich: Instructions for use
Imegen-Friedreich: MSDS
Analysis certificate

Imegen-Friedreich

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