Detection of the *27 allele of the HLA-B gene, associated with autoimmune inflammatory diseases of the spine, spine and sacroiliac joints

48 rxn

Real-time PCR


7500 FAST Real-Time PCR System (ThermoFisher), StepOne Real-Time PCR System (Thermo Fisher), CFX96 Real-Time PCR System (BioRad)

  • Multiplex assay that allows amplifying the different subtypes of the B27 allele, located in exon 2 of the HLA-B gene, offering high sensitivity and specificity.
  • The purpose of the Imegen-HLA B27 kit is not to determine tissue typing for histocompatibility testing prior to transplantation.
  • It includes a positive control that guarantees the functionality of the PCR system and that helps the interpretation in the qualitative analysis.
  • Includes an endogenous control to confirm sample quality and integrity
  • For in vitro diagnostic use.

Qualitative real-time PCR assay employing a combination of oligonucleotides and fluorescent hydrolysis probes in a validated assay to simultaneously detect the presence of the *27 allele of the HLA-B gene and the endogenous β-globin gene.

  • Amount of DNA needed: 50 ng total
  • Type of sample: Peripheral blood (Germinal) or saliva
  • Number of reactions per sample: 1
  • Number of targets: 1
  • Manual working time: 30 min
  • Duration of the PCR program: 1h
  • Compatible cyclers: real-time PCR cycler with FAMTM and VIC® channels


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