Determination of the number of copies of the rearrangement PML-RARA t(15;17)(q22;q21) type bcr1 associated with Acute Promyelocytic Leukemia (APL)

48×2 rxn (dehydrated/lyophilized)

Real-time PCR


7500 FAST Real-Time PCR System (ThermoFisher), StepOnePlus Real-Time PCR System (ThermoFisher)

  • Quantitative assay to determine the number of copies of the PML-RARA t(15;17)(q22;q21) rearrangement type bcr1 and the number of copies of the endogenous GUS gene.
  • Allows detection of minimal residual disease (MD). The theoretical sensitivity level that can be achieved is MR4 (0.01% of PML-RARE).
  • High sensitivity and specificity: The limit of quantification (LOQ) has been set at 125 total copies for both the GUS reference gene and the PML-RARA rearrangement.
  • It includes an endogenous control of the GUS gene to be able to carry out the relative quantification. In addition, it allows to confirm the quality and integrity of the sample.
  • For use in research.

Real-time PCR analysis employing a combination of oligonucleotides and fluorescent hydrolysis probes for relative quantification of PML-RARA bcr1 rearrangement copy number relative to GUS reference gene copy number.

  • Amount of cDNA required: 20µL of cDNA resulting from the reverse transcription of 1 µg of RNA
  • Sample type: Peripheral blood
  • Number of reactions per sample: 4
  • Number of targets: 2
  • Manual working time: 45 min
  • Duration of the PCR program: 1h 15 min
  • Compatible cyclers: Real-time PCR cycler with the FAMTM


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