- Multiplexed assay: allows to determine the presence of 16 polymorphic markers in recipient and donor samples for the selection of an informative marker for patient follow-up.
- High sensitivity and specificity: The targets are polymorphic markers of null alleles, insertions or deletions.
- For the quantification of molecular chimerism, SRY or RhD can also be used if they are positive in the recipient and negative in the donor, knowing the gender and/or blood group of the recipient and the donor.
- Cumulative global informativeness together with SRY and RhD of 96% in Caucasian population.
- It complies with the quality requirements specified by ISO 13485 and ISO 14001 in relation to the materials used in its manufacturing process.
- For research use.
Description
Informative molecular marker screening for real-time PCR monitoring of allogeneic bone marrow transplant patients
Format
10 rxn (5 casos de trasplante) -LIOFILIZADO/DESHIDRATADO
Technology
Real-time PCR
Reference
IMG-116-24
Compatible equipment
7500 FAST Real-Time PCR System (ThermoFisher)
Real-time multiplexed PCR uses a system of sequence-specific oligonucleotides and hydrolysis probes that allow targets of interest to be specifically amplified.
- Amount of DNA needed: 450 ng of recipient DNA sample (pre-transplant) and 450 ng of donor DNA sample
- Sample type: Peripheral blood or bone marrow
- Number of reactions per recipient sample: 8
- Number of reactions per donor sample: 8
- Number of targets: 16
- Manual working time: 45 min
- Duration of the PCR program: 1h 20 min
- Compatible cyclers: real-time PCR cycler with FAMTM and VIC® channels.
| Reference | Kit name | Marker |
| IMG-116-2,IMG-116-18,IMG-116-3,IMG-116-4,IMG-116-5,IMG-116-6,IMG-116-7,IMG-116-8,IMG-116-9,IMG-116-10,IMG-116-11,IMG-116-12,IMG-116-13,IMG-116-14,IMG-116-17,IMG-116-20,IMG-116-21,IMG-116-23 | Imegen-Quimera SRY,Imegen-Quimera RhD,Imegen-Quimera Q116-3I,Imegen-Quimera Q116-4I,Imegen-Quimera Q116-5I,Imegen-Quimera Q116-6I,Imegen-Quimera Q116-7I,Imegen-Quimera Q116-8I,Imegen-Quimera Q116-9I,Imegen-Quimera Q116-10I,Imegen-Quimera Q116-11I ,Imegen-Quimera Q116-12I,Imegen-Quimera Q116-4D,Imegen-Quimera Q116-5D,Imegen-Quimera Q116-10D,Imegen-Quimera Q116-20I,Imegen-Quimera Q116-12D,Imegen-Quimera Q116-23I | SRY,RhD,Q116-3I,Q116-4I,Q116-5I,Q116-6I,Q116-7I,Q116-8I,Q116-9I,Q116-10I,Q116-11I,Q116-12I,Q116-4D,Q116-5D,Q116-10D,Q116-20I,Q116-12D,Q116-23I |
Related kits
Imegen-Quimera Screening Multiplex I
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