- Multiplexed assay: allows to determine the presence of 16 polymorphic markers in recipient and donor samples for the selection of an informative marker for patient follow-up.
- High sensitivity and specificity: The targets are polymorphic markers of null alleles, insertions or deletions.
- For the quantification of molecular chimerism, SRY or RhD can also be used if they are positive in the recipient and negative in the donor, knowing the gender and/or blood group of the recipient and the donor.
- Cumulative global informativeness together with SRY and RhD of 96% in Caucasian population.
- It complies with the quality requirements specified by ISO 13485 and ISO 14001 in relation to the materials used in its manufacturing process.
- For research use.
Description
Informative molecular marker screening for real-time PCR monitoring of allogeneic bone marrow transplant patients
Format
10 rxn (5 casos de trasplante) -LIOFILIZADO/DESHIDRATADO
Technology
Real-time PCR
Reference
IMG-116-24
Compatible equipment
7500 FAST Real-Time PCR System (ThermoFisher)
Real-time multiplexed PCR uses a system of sequence-specific oligonucleotides and hydrolysis probes that allow targets of interest to be specifically amplified.
- Amount of DNA needed: 450 ng of recipient DNA sample (pre-transplant) and 450 ng of donor DNA sample
- Sample type: Peripheral blood or bone marrow
- Number of reactions per recipient sample: 8
- Number of reactions per donor sample: 8
- Number of targets: 16
- Manual working time: 45 min
- Duration of the PCR program: 1h 20 min
- Compatible cyclers: real-time PCR cycler with FAMTM and VIC® channels.
| Reference | Kit name | Marker |
| IMG-116-3 | Imegen-Quimera Q116-3I | Q116-3I |
| IMG-116-4 | Imegen-Quimera Q116-4I | Q116-4I |
| IMG-116-5 | Imegen-Quimera Q116-5I | Q116-5I |
| IMG-116-6 | Imegen-Quimera Q116-6I | Q116-6I |
| IMG-116-7 | Imegen-Quimera Q116-7I | Q116-7I |
| IMG-116-8 | Imegen-Quimera Q116-8I | Q116-8I |
| IMG-116-9 | Imegen-Quimera Q116-9I | Q116-9I |
| IMG-116-10 | Imegen-Quimera Q116-10I | Q116-10I |
| IMG-116-11 | Imegen-Quimera Q116-11I | Q116-11I |
| IMG-116-12 | Imegen-Quimera Q116-12I | Q116-12I |
| IMG-116-13 | Imegen-Quimera Q116-4D | Q116-4D |
| IMG-116-14 | Imegen-Quimera Q116-5D | Q116-5D |
| IMG-116-17 | Imegen-Quimera Q116-10D | Q116-10D |
| IMG-116-20 | Imegen-Quimera Q116-20I | Q116-20I |
| IMG-116-21 | Imegen-Quimera Q116-12D | Q116-12D |
| IMG-116-23 | Imegen-Quimera Q116-23I | Q116-23I |
Related kits
Imegen-Quimera Screening Multiplex I
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