Imegen-Quimera Screening Multiplex I

Informative molecular marker screening for real-time PCR monitoring of allogeneic bone marrow transplant patients

10 rxn (5 casos de trasplante) -LIOFILIZADO/DESHIDRATADO

Real-time PCR


7500 FAST Real-Time PCR System (ThermoFisher)

  • Multiplexed assay: allows to determine the presence of 16 polymorphic markers in recipient and donor samples for the selection of an informative marker for patient follow-up.
  • High sensitivity and specificity: The targets are polymorphic markers of null alleles, insertions or deletions.
  • For the quantification of molecular chimerism, SRY or RhD can also be used if they are positive in the recipient and negative in the donor, knowing the gender and/or blood group of the recipient and the donor.
  • Cumulative global informativeness together with SRY and RhD of 96% in Caucasian population.
  • For research use.

Imegen-Quimera Screening Multiplex IReal-time multiplexed PCR uses a system of sequence-specific oligonucleotides and hydrolysis probes that allow targets of interest to be specifically amplified.

  • Amount of DNA needed: 450 ng of recipient DNA sample (pre-transplant) and 450 ng of donor DNA sample
  • Sample type: Peripheral blood or bone marrow
  • Number of reactions per recipient sample: 8
  • Number of reactions per donor sample: 8
  • Number of targets: 16
  • Manual working time: 45 min
  • Duration of the PCR program: 1h 20 min
  • Compatible cyclers: real-time PCR cycler with FAMTM and VIC® channels.
Reference Kit name Marker
IMG-116-3 Imegen-Quimera Q116-3I Q116-3I
IMG-116-4 Imegen-Quimera Q116-4I Q116-4I
IMG-116-5 Imegen-Quimera Q116-5I Q116-5I
IMG-116-6 Imegen-Quimera Q116-6I Q116-6I
IMG-116-7 Imegen-Quimera Q116-7I Q116-7I
IMG-116-8 Imegen-Quimera Q116-8I Q116-8I
IMG-116-9 Imegen-Quimera Q116-9I Q116-9I
IMG-116-10 Imegen-Quimera Q116-10I Q116-10I
IMG-116-11 Imegen-Quimera Q116-11I Q116-11I
IMG-116-12 Imegen-Quimera Q116-12I Q116-12I
IMG-116-13 Imegen-Quimera Q116-4D Q116-4D
IMG-116-14 Imegen-Quimera Q116-5D Q116-5D
IMG-116-17 Imegen-Quimera Q116-10D Q116-10D
IMG-116-20 Imegen-Quimera Q116-20I Q116-20I
IMG-116-21 Imegen-Quimera Q116-12D Q116-12D
IMG-116-23 Imegen-Quimera Q116-23I Q116-23I

Imegen-Quimera Screening Multiplex I

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