Imegen-Quimera Screening Multiplex II

Informative molecular marker screening for real-time PCR monitoring of allogeneic bone marrow transplant patients

10 rxn (5 transplant cases) -LYOPHILIZED/DEHYDRATED

Real Time PCR

IMG-116-74

  • Multiplexed assay: allows to determine the presence of 16 polymorphic markers, each one, in recipient and donor samples for the selection of an informative marker for patient follow-up.
  • High sensitivity and specificity: The targets are polymorphic markers of null alleles, insertions or deletions.
  • For the quantification of molecular chimerism, SRY or RhD can also be used if they are positive in the recipient and negative in the donor, knowing the gender and/or blood group of the recipient and the donor.
  • Accumulated global informativity together with SRY and RhD of 98.8% in Caucasian population.
  • It complies with the quality requirements specified by ISO 13485 and ISO 14001 regarding the materials used in its manufacturing process.
  • For in vitro diagnostic use.

Real-time multiplexed PCR uses a system of sequence-specific oligonucleotides and hydrolysis probes that allow targets of interest to be specifically amplified.

  • Amount of DNA needed: 450 ng of recipient DNA sample (pre-transplant) and 450 ng of donor DNA sample
  • Type of sample: Peripheral blood or bone marrow
  •  Number of reactions per recipient sample: 8
  •  Number of reactions per donor sample: 8
  • <li”>Number of targets: 16
  • Manual work time: 45 min
  • Duration of the PCR program: 1h 20 min
  • Compatible cyclers: real-time PCR cycler with FAMTM and VIC® channels

Imegen-Quimera Screening Multiplex II

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