Imegen-Quimera Screening Multiplex Plus II

Informative molecular marker screening for digital PCR follow-up of allogeneic bone marrow transplant patients

10 rxn (5 transplant cases) -LYOPHILIZED/DEHYDRATED

Real-time PCR

IMG-116-25

7500 FAST Real-Time PCR System (ThermoFisher), StepOnePlus Real-Time PCR System (ThermoFisher), LightCycler 480 (Roche)

  • Multiplexed assays: they allow to determine the presence of 16 polymorphic markers, each one, in recipient and donor samples for the selection of an informative marker for patient follow-up.
  • High sensitivity and specificity: The targets are polymorphic markers of null alleles, insertions or deletions.
  • For the quantification of molecular chimerism, SRY or RhD can also be used if they are positive in the recipient and negative in the donor, knowing the gender and/or blood group of the recipient and the donor.
  • Cumulative global informativeness of the 33 markers together with SRY of 99.98% in the Caucasian population.
  • It complies with the quality requirements specified by ISO 13485 and ISO 14001 in relation to the materials used in its manufacturing process.
  • For in vitro diagnostic use.

Imegen-Quimera Screening Multiplex Plus IIReal-time multiplexed PCR uses a system of sequence-specific oligonucleotides and hydrolysis probes that allow targets of interest to be specifically amplified.

  • Amount of DNA needed: 450 ng of recipient DNA sample (pre-transplant) and 450 ng of donor DNA sample
  • Sample type: Peripheral blood or bone marrow
  • Number of reactions per recipient sample: 8
  • Number of reactions per donor sample: 8
  • Number of targets: 16
  • Manual working time: 45 min
  • Duration of the PCR program: 1h 20 min
  • Compatible cyclers: real-time PCR cycler with FAMTM and VIC® channels
Reference Kit name Marker
IMG-116-28 Imegen-Quimera RhD dPCR RhD
IMG-116-40 Imegen-Quimera Q116-20I dPCR Q116-20I
IMG-116-47 Imegen-Quimera Q116-33I dPCR Q116-33I
IMG-116-48 Imegen-Quimera Q116-9I dPCR Q116-9I
IMG-116-49 Imegen-Quimera Q116-37I dPCR Q116-37I
IMG-116-50 Imegen-Quimera Q116-38I dPCR Q116-38I
IMG-116-51 Imegen-Quimera Q116-39I dPCR Q116-39I
IMG-116-52 Imegen-Quimera Q116-41I dPCR Q116-41I
IMG-116-53 Imegen-Quimera Q116-42I dPCR Q116-42I
IMG-116-54 Imegen-Quimera Q116-43I dPCR Q116-43I
IMG-116-55 Imegen-Quimera Q116-44I dPCR Q116-44I
IMG-116-56 Imegen-Quimera Q116-45I dPCR Q116-45I
IMG-116-57 Imegen-Quimera Q116-47I dPCR Q116-47I
IMG-116-58 Imegen-Quimera Q116-49I dPCR Q116-49I
IMG-116-59 Imegen-Quimera Q116-50I dPCR Q116-50I
IMG-116-60 Imegen-Quimera Q116-46I dPCR Q116-46I

Imegen-Quimera Screening Multiplex Plus II

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