- Multiplexed assays: they allow to determine the presence of 16 polymorphic markers, each one, in recipient and donor samples for the selection of an informative marker for patient follow-up.
- High sensitivity and specificity: The targets are polymorphic markers of null alleles, insertions or deletions.
- For the quantification of molecular chimerism, SRY or RhD can also be used if they are positive in the recipient and negative in the donor, knowing the gender and/or blood group of the recipient and the donor.
- Cumulative global informativeness of the 33 markers together with SRY of 99.98% in the Caucasian population.
- It complies with the quality requirements specified by ISO 13485 and ISO 14001 in relation to the materials used in its manufacturing process.
- For in vitro diagnostic use.
Description
Informative molecular marker screening for digital PCR follow-up of allogeneic bone marrow transplant patients
Format
10 rxn (5 transplant cases) -LYOPHILIZED/DEHYDRATED
Technology
Real-time PCR
Reference
IMG-116-25
Compatible equipment
7500 FAST Real-Time PCR System (ThermoFisher), StepOnePlus Real-Time PCR System (ThermoFisher), LightCycler 480 (Roche)
Real-time multiplexed PCR uses a system of sequence-specific oligonucleotides and hydrolysis probes that allow targets of interest to be specifically amplified.
- Amount of DNA needed: 450 ng of recipient DNA sample (pre-transplant) and 450 ng of donor DNA sample
- Sample type: Peripheral blood or bone marrow
- Number of reactions per recipient sample: 8
- Number of reactions per donor sample: 8
- Number of targets: 16
- Manual working time: 45 min
- Duration of the PCR program: 1h 20 min
- Compatible cyclers: real-time PCR cycler with FAMTM and VIC® channels
| Reference | Kit name | Marker |
| IMG-116-28 | Imegen-Quimera RhD dPCR | RhD |
| IMG-116-40 | Imegen-Quimera Q116-20I dPCR | Q116-20I |
| IMG-116-47 | Imegen-Quimera Q116-33I dPCR | Q116-33I |
| IMG-116-48 | Imegen-Quimera Q116-9I dPCR | Q116-9I |
| IMG-116-49 | Imegen-Quimera Q116-37I dPCR | Q116-37I |
| IMG-116-50 | Imegen-Quimera Q116-38I dPCR | Q116-38I |
| IMG-116-51 | Imegen-Quimera Q116-39I dPCR | Q116-39I |
| IMG-116-52 | Imegen-Quimera Q116-41I dPCR | Q116-41I |
| IMG-116-53 | Imegen-Quimera Q116-42I dPCR | Q116-42I |
| IMG-116-54 | Imegen-Quimera Q116-43I dPCR | Q116-43I |
| IMG-116-55 | Imegen-Quimera Q116-44I dPCR | Q116-44I |
| IMG-116-56 | Imegen-Quimera Q116-45I dPCR | Q116-45I |
| IMG-116-57 | Imegen-Quimera Q116-47I dPCR | Q116-47I |
| IMG-116-58 | Imegen-Quimera Q116-49I dPCR | Q116-49I |
| IMG-116-59 | Imegen-Quimera Q116-50I dPCR | Q116-50I |
| IMG-116-60 | Imegen-Quimera Q116-46I dPCR | Q116-46I |
Imegen-Quimera Screening Multiplex Plus II
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