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NPM1 dPCR OncoKit

Description

Detection and quantification of NPM1 gene pathogenic variants (A, B and D) associated with acute myeloid leukemia (AML) in peripheral blood complementary DNA (cDNA) samples

Format

24 rxn (dehydrated/freeze-dried)

Technology

Digital PCR (dPCR)

Reference

IMG-432

Compatible equipment

ThermoFisher

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Characteristics
Technology
Technical specifications
Documentation

Quantitative multiplex assay that allows the detection and quantification of NPM1 gene variants causing acute myeloid leukemia (AML) in peripheral blood complementary DNA (cDNA).
NPM1 dPCR OncoKit is intended for use in research and is aimed exclusively at healthcare workers and professionals working in molecular biology.

NPM1 dPCR OncoKit is designed to detect and quantify NPM1 gene variants A, B or D associated with acute myeloid leukemia through digital PCR (dPCR), a highly sensitive, precise, and accurate end-point PCR method that allows absolute quantification of a sample that is subdivided into 20,000 partitions.

The kit employs a combination of oligonucleotides and fluorescent hydrolysis probes in a competitive multiplex assay that allows the detection and relative quantification of the fusion of interest in the sample.

Type of sample: cDNA from peripheral blood
Positive control: DNA probe with the pathogenic variants (A, B and D) and a wild-type NPM1 region
Limit of detection: 0.04% ± 0.012
Limit of quantification: 0.1%
Number of reactions per sample: 1
Number of targets: 4 (A, B and D variants in NPM1 and a wild-type NPM1 region serving as an endogen)
dPCR program duration: 1 h 45 min.

NPM1 dPCR OncoKit: MSDS

NPM1 dPCR OncoKit: Instructions for use

Analysis certificate

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