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Redefining
diagnosis
in every read.
Revio™
Unleash the power of HiFi sequencing.
Accuracy, scalability, and simplicity
in your hands.
LONG-READ SEQUENCING
A new paradigm
PacBio’s long-read sequencing is a sequencing technology that yields genomic data by generating individual reads between 1,000 and 20,000 nucleotides long, derived from a single DNA molecule.
These fragments come from DNA molecules directly extracted from the biological sample, while other mass sequencing technologies require synthetically copying DNA before analysis.
LONG-READ VS. SHORT-READ
Tell me what you read
and I’ll tell you who you are.
HiFi reads with
accuracy
Long-read technologies, such as PacBio HiFi sequencing, are quickly becoming the new gold standard in genomics research.
With Hi-Fi readings, it is no longer necessary to complement the advantages of long reads with the accuracy of short reads. This technology combines the best of both, allowing for accurate sequencing using long fragments, which is ideal for addressing the most complex biological questions.
HiFi reads
Higher read
quality.
Long-reads
Genomic
assembly with a
higher accuracy.
Not all readings
are fairytales.
| HiFi1,2 | Short-read3 | |
|---|---|---|
| Read length | 1-25 Kb | 50-300 bp |
| Read accuracy | 99,95% | 99,99% |
| Yield | 90 Gb4,5 | Up to 8 Tb |
| Small variants (SNVs + in/dels) |  |
|
| Structural variants (SVs) | |
 |
| Tandem repeats | |
|
| Segment duplications | |
|
| Pseudogenes | |
|
| Methylation | |
|
| Phasing | |
|
(1) Ebert et al. (2021) Science 372(6537):eabf7117
(2) Cohen et al. (2022) Genetic Med doi:10.1016/j.gim.2022.02.007
(3) Byrska-Bishop et al. (2021) bioRxiv doi:10.1101/2021.02.06.430068
(4) The specified HiFi yield is based on HG002/GM24385 human DNA extracted using the Nanobind CBB kit and prepared using the SMRTbell prep 3.0 kit.
(5) HiFi yield depends on library fragment size. Yield is usually lower for shorter libraries.
Revio™.
A giant leap.
PacBio’s Revio platform, thanks to its HiFi technology, expands data yield up to 15-fold with respect to previous PacBio platforms and allows reducing the use of consumables by up to 50%.
The platform has four independent sequencing modules that allow for the simultaneous sequencing of four SMRT cells. Each one of these SMRT cells allows studying one human genome at 30X depth.
Read lengths up to 25 kb and a high accuracy (>99.9 %) make Revio the ideal option for a wide range of genomic applications, including de novo assembly, comprehensive variant detection, transcriptomics of individual cells, and epigenetic characterization.
Uniform coverage.
No bias even for high GC-content regions.
Flexible scalability.
From individual genes to whole genomes.
Longer average
read lengths.
Up to 25-Kb long.
Higher consensus accuracy (HiFi reads).
Minimizes sequencing errors associated with long reads.
Epigenetic characterization.
No need for bisulfite conversion.
Uniform coverage.
Even in hard-to-sequence
or hard-to-align regions.
Haplotype resolution.
It allows differentiating variants in each of the copies of a gene.
It opens the door into a more comprehensive view of biology.
Health in Code is PacBio’s official service provider in southern Europe, supporting small- and large-scale HiFi long-read projects.
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