Home / Analysis kits / Hereditary OncoKitDx (without index)

Hereditary OncoKitDx (without index)

Description

NGS sequencing of 50 genes associated with hereditary cancer predisposition

Format

48 rxn (Dilution)

Technology

NGS

Reference

IMG-326

Compatible equipment

Illumina MiSeq, NextSeq 500/550Dx System

Kit Health in Code
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  • Dynamic kit that updates the target genes of the panel according to scientific evidence.
  • Type of mutations analyzed: SNVs, INDELs, CNVs, ALUs and other large insertions.
  • Coverage: 99.5% of bases covered at a depth of 50X.
  • Uniformity: 99.2% of the bases covered at >20% of the average coverage and 90.0% of the bases covered at >50% of the average coverage.
  • Sensitivity: > 99.9%
  • Specificity: > 99.9%
  • Necessary reagents to perform libraries indexing reactions are not included
  • For in vitro diagnostic use.

Hereditary OncoKitDx (without index) employs library preparation technology by enzymatic fragmentation and enrichment of regions of interest by hybridization with capture probes. Subsequently, massive sequencing is carried out on Illumina platforms.

Compatible sequencers Illumina MiSeq, NextSeq 500/550Dx System
Number of reactions 48
Number of samples per run Illumina Miseq Micro (300 ciclos): 12

Illumina Miseq V2 (300 ciclos): 48
Sequencing: Paired-end (2 x 150)
Amount of DNA per sample 20-26 ng
Size of regions of interest 184 Kb
Target genes 50 genes: APC, ATM, BARD1, BMPR1A, BRCA1, BRCA2, BRIP1, CDH1, CDK4, CDKN2A, CHEK2, EPCAM, FAM175A(ABRAXAS1), FH, KIF1B, MAX, MEN1, MET, MLH1, MLH3, MRE11, MSH2, MSH3, MSH6, MUTYH, NBN, NF1, NTHL1, PALB2, PIK3CA, PMS2, POLD1, POLE, PTEN, RAD50, RAD51C, RAD51D, RB1, RET, SDHA, SDHAF2, SDHB, SDHC, SDHD, SMAD4, STK11, TMEM127, TP53, VHL, XRCC2
Type of sample Blood or Saliva
Hereditary OncoKitDx (without index): Ratio of genes by tumour
Hereditary OncoKitDx (without index): Workflow
Hereditary OncoKitDx (without index): MSDS
Hereditary OncoKitDx (without index): Instructions for use
Analysis certificate

Hereditary OncoKitDx (without index)

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