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Action ST OncoKit

Protocol automated using Magnis NGS Prep System

Description

Testing for main genomic biomarkers with clinical value in the diagnosis, prognosis, sensitivity and/or therapeutic resistance of a wide range of solid tumors, such as lung, breast, and colorectal cancer, among others, by NGS

Format

24 rxn

Technology

NGS sequencing

Reference

IMG-419

Compatible equipment

NextSeq 500/550Dx System

Protocol automated using Magnis NGS Prep System
Kit Health in Code
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  • It detects point mutations (SNVs), small insertions and deletions (INDELs), structural variants (SVs), copy number variants (CNVs) throughout the genome, microsatellite instability (MSI) and pharmacogenetics variants (PGx).
  • UMI molecular barcoding to increase the sensitivity of the bioinformatics analysis.
  • Included analysis software: Data Genomics.
  • Coverage: >99% of bases covered at 200X.
  • Uniformity: 98% of bases covered at >20% of mean coverage.
  • Sensitivity: >99%.
  • Specificity: >99%.
  • Limit of detection (LOD) for SNVs, INDELs, and structural variants is 5%.
  • Limit of detection for CNVs is 2.4 total copies for copy number gains, which means 4 copies at 30% of tumor burden, and 1.8 total copies for copy number losses, which is 1 copy at 20% of tumor burden.
Action ST OncoKit
  • Compatible sequencers: NextSeq 500/550Dx System
  • Number of reactions: 24
  • Number of samples per run: Mid Output v2.5 kit (150 cycles): 8 samples
  • Sequencing: Paired-end (2 x 75 cycles)
  • Amount of DNA per sample: 10-200 ng
  • Analyzed genomic region: 500 Kb
  • Sample type: DNA from peripheral blood or paraffin-embedded tissue
Action ST OncoKit: MSDS
Action ST OncoKit: Instructions for use
Analysis certificate

Action ST OncoKit

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