Detection of INDEL-type mutations in the CALR gene associated with Myeloproliferative Neoplasms (MPN)

33 rxn

Fragment Analysis


3730xl DNA Analyzer (ThermoFisher Scientific)

  • Qualitative analysis that evaluates insertion or deletion mutations (INDELs) in exon 9 of the CALR gene in relation to the size of the amplification fragments obtained.
  • Amplification range of 100-500 bp.
  • According to the specified protocol, the detection limit has been established at 5%.
  • It includes a positive control at the optimal amplification concentration, in which a normal allele is present, the most frequent 5-base insertion and 52-base deletion variants, as well as a single-base deletion allele demonstrating power. technique discrimination.
  • For in vitro diagnostic use.

Imegen-CALR allows analysis of insertions/deletions in exon 9 in the CALR gene, by means of PCR and subsequent capillary electrophoresis.

  • Amount of DNA: 50 ng
  • Sample type: Sangre periférica (Germinal)
  • Number of reactions per sample: 1
  • Number of targets: 1
  • Number of targets: 30 minutos
  • Number of targets: 1h 45 min
  • Compatible thermal cyclers: Compatible thermal cyclers
  • Sequencing with polymers compatible with the label 6-Carboxyfluorescein (6-FAM)


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