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Imegen-Cambridge II

Description

Detection of the c.1246G>T (p.Ala416Ser) allele in the SERPINC1 gene associated with Cambridge II Syndrome

Format

48 rxn

Technology

Real-time PCR

Reference

IMG-199

Compatible equipment

7500 FAST Real-Time PCR System (ThermoFisher), StepOnePlus Real-Time PCR System (ThermoFisher), StepOne Real-Time PCR System (Thermo Fisher)

Kit Health in Code
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  • Real-time PCR assay allows determining the presence of the normal allele or the pathogenic allele p.Ala416Ser (NM000488.3:c.1246G>T) in the SERPINC1 gene associated with Cambridge II disease, in a single PCR reaction.
  • High sensitivity and specificity: Discriminates in 100% of the samples the normal allele from the mutated allele associated with the Cambridge II variant.
  • Validated using the same real-time PCR program as the products related to alterations in the coagulation process, so they can be analyzed together.
  • For research use only, aimed at professionals in the molecular biology industry.

Imegen-Cambridge II employs a combination of oligonucleotides and fluorescent hydrolysis probes in a real-time PCR assay to specifically discriminate targets of interest in a single PCR reaction.

  • Amount of DNA needed: 50 ng total
  • Sample type: peripheral (germ) blood, saliva or buccal smear
  • Number of reactions per sample: 1
  • Number of targets: 2
  • Manual working time: 30 min
  • Duration of the PCR program: 1h 15 min
  • Compatible cyclers: real-time PCR cycler with FAMTM and VIC®
Imegen-Cambridge II: Manual
Imegen-Cambridge II: MSDS
Analysis certificate

Imegen-Cambridge II

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