Detection of subtype *57:01 of the HLA-B gene associated with hypersensitivity to Abacavir

48 rxn

Real-time PCR


7500 FAST Real-Time PCR System (ThermoFisher), StepOne Real-Time PCR System (Thermo Fisher)

  • Multiplex assay that allows amplifying the *57:01 subtype of the HLA-B gene, offering high sensitivity and specificity.
  • It includes a positive control that guarantees the functionality of the PCR system and that helps the interpretation in the qualitative analysis.
  • Includes an endogenous control to confirm sample quality and integrity.
  • For in vitro diagnostic use.

Imegen-HLA-B57:01 Qualitative real-time PCR assay employing a combination of oligonucleotides and fluorescent hydrolysis probes in a validated assay to simultaneously detect the presence of the HLA-B gene *57:01 subtype and the endogenous β-globin gene.

  • Amount of DNA needed: 50 ng total
  • Type of sample: Peripheral blood (Germinal) and saliva
  • Number of reactions per sample: 1
  • Number of targets: 1
  • Manual working time: 30 min
  • Duration of the PCR program: 1h
  • Compatible cyclers: real-time PCR cycler with FAMTM and VIC® channels


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