Imegen-Huntington

Detection of CAG expansion in the HTT gene associated with Huntington's disease (HD)

12 rxn

Fragment Analysis

IMG-154

3730xl DNA Analyzer (ThermoFisher Scientific)

  • Semi-quantitative analysis of the number of repetitions of the CAG expansion of the HTT gene by means of two PCRs (HTT-C and HTT-L).
  • Amplification range of 100-500 bp.
  • Includes a positive control at the optimal amplification concentration, in which normal alleles are present for all assays performed with the kit, to demonstrate the functionality of the prepared amplification reactions.
  • Designed and validated with the same PCR program as related products for the analysis of expansions involved in other neurodegenerative diseases, so they can be analyzed together.
  • For in vitro diagnostic use.

Imegen-Huntington allows analysis of CAG triplet expansions in the HTT gene associated with Huntington’s disease by conventional PCR and capillary electrophoresis.

  • Amount of DNA needed: 50 ng for each amplification system
  • Sample type: Peripheral blood (Germinal)
  • Number of reactions per sample: 2
  • Number of targets: 2 (CAG and poly-CCG)
  • Manual work time: 45 minutes
  • Duration of the PCR program: 2 h 15 min
  • Compatible thermal cyclers: Any conventional thermal cycler
  • Sequencing with compatible polymers with 6-Carboxyfluorescein (6-FAM) and NEDTM.

Imegen-Huntington

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