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Liquid Biopsy – Targeted Liquid Biopsy OncoKit
Analysis of tumors using liquid biopsy.
Characteristics:
- NGS panel and analysis software to screen for the study of the main biomarkers of clinical interest in non-small cell lung cancer and other solid tumors.
- Characterization of ctDNA obtained from a liquid biopsy sample.
- SNV, indel, genetic fusion, and CNV detection in a single DNA sample.
- Bioinformatic analysis with the Data Genomics software and semi-automated generation of reports, which include both functional and clinical classification of the variants.
- Based on high sensitivity RNA-probe technology with UMI barcoding (unique molecular identifiers).
- Mean coverage: 7300X, with mean coverage after UMI analysis: 2500X.
- The specificity, sensitivity, and repeatability of this kit are >99% and reproducibility is >97%. Coverage (at a read depth >100X) and uniformity of bases covered at >20X is >96%.
- Limit of detection: SNVs, indels and structural variants: 1%; CNVs: gains of 2.9 copies.
- Compliant with ISO 9001 and ISO 14001 quality specifications regarding manufacturing materials.
- CE-IVD-marked analysis software.
Turnaround time (TAT): 12 working days
Ref. S-202212577
- Compatible sequencers: Illumina NextSeq500/550Dx.
- Number of reactions: 24.
- Number of samples per run: 8 samples in a NextSeq HIGH output cartridge (150 cycles).
- Sequencing: Paired-end (2 x 75 cycles).
- Sample type: Circulating cell-free DNA from a liquid biopsy sample.
- Amoung of input DNA: 10-50 ng.
- Panel fully automated on Magnis NGS Prep System Dx.
- CNV analysis requires that a positive control (REF: IMG-368) be used at least once.
– Sequencing of whole exonic regions of 13 genes:
- ALK
- BRAF
- EGFR
- ERBB2
- KIT
- KRAS
- MAP2K1
- MET
- NRAS
- PIK3CA
- RB1
- ROS1
- TP53
– Sequencing of 7 hotspot regions in the NTRK1 and NTRK3 genes.
– Capture of 8 fusion genes with all possible rearrangements covered (including intronic regions with breakpoints previously reported in the literature):
- ALK
- EGFR
- ETV6
- NRG1
- NTRK1
- NTRK2
- RET
- ROS1
– Detection of CNVs across the whole genome. Validation of results and detection of copy-neutral LOH through a low-density SNP array spanning the whole genome.
Steps to follow
How to order
1. Download & fill out
Please cover as many fields as possible in both documents
2. Sample collection
Three sample types: saliva, peripheral blood or genomic DNA
3. Pack the sample
Please pack the sample in a way to prevent leakage
4. Send the sample & the request
Please schedule the delivery for Monday–Friday: 8am – 5pm
5. Result: the report
Via: e-mail and/or through the customer portal
Ask us for more information
Liquid Biopsy – Targeted Liquid Biopsy OncoKit
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