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FLT3-TKD2 dPCR OncoKit

Description

Detection and quantification of variants associated with acute myeloid leukemia (AML) at codons 835 and 836 of the FLT3 gene in genomic DNA sample

Format

24 rxn (dehydrated/freeze-dried)

Technology

Digital PCR (dPCR)

Reference

IMG-405

Compatible equipment

QuantStudio^TM Absolute Q^TM Digital PCR System (ThermoFisher Scientific)

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Characteristics
Technology
Technical specifications
Documentation

Quantitative multiplex assay: quantification of mutations at codons D835 and I836, located in the TKD2 domain of FLT3, in genomic DNA from peripheral blood by means of digital PCR (dPCR).
FLT3-TKD2 dPCR OncoKit is intended for use in research and is aimed exclusively at healthcare workers and professionals working in molecular biology.

FLT3-TKD2 dPCR OncoKitis designed to quantify pathogenic variant at codons D835 and I836 of the second tyrosine kinase domain (TKD2) of the FLT3 gene. The kit utilizes digital PCR (dPCR), a highly sensitive, precise, and accurate end-point PCR method that allows absolute quantification of a sample that is subdivided into 20,000 partitions.

The kit employs a combination of oligonucleotides and fluorescent hydrolysis probes in a non-competitive multiplex assay for quantification of the alternative allele.

Sample type: Genomic DNA from peripheral blood
Positive control: Synthetic DNA with heterozygous FLT3 p.D835Y
Necessary amount of DNA: 65 ng.
Limit of detection: 0,2% ± 5,4E-4.
Limit of quantification: 0,5%
Number of reactions per sample: 1
Number of targets: 2 (altered allele and unchanged, wild-type, allele of FLT3).
Duration of the dPCR program: 1 h 45 min.

FLT3-TKD2 dPCR OncoKit: Instructions for use

FLT3-TKD2 dPCR OncoKit:

Analysis certificate

Related kits

Imegen-FLT3

IMG-238