Determination of the number of copies of the CBFB-MYH11 type A rearrangement related to Acute Myeloid Leukemia (AML)

48×2 rxn

real-time PCR


7500 FAST Real-Time PCR System (ThermoFisher), StepOnePlus Real-Time PCR System (ThermoFisher)

  • Quantitative assay to determine the number of copies of the CBFB-MYH11 Type A rearrangement resulting from the pericentric inversion of chromosome 16 [inv(16)(p13q22)] and the number of copies of the endogenous ABL1 gene.
  • It includes an endogenous control of the ABL1 gene to be able to carry out the relative quantification. In addition, it allows to confirm the quality and integrity of the sample.
  • Allows detection of minimal residual disease (MD). The theoretical sensitivity level that can be achieved is MR 4 (0.01% of inv(16)(p13q22)).
  • High sensitivity and specificity: The limit of quantification (LOQ) has been set at 125 total copies for both theABL1reference gene and the inv(16)(p13q22) rearrangement.
  • For research use.

Real-time PCR assay employing a combination of oligonucleotides and fluorescent hydrolysis probes for relative quantification of CBFB-MYH11 Type A rearrangement copy number relative to ABL1 reference gene copy number.

  • Amount of cDNA required: 20µL of cDNA resulting from the reverse transcription of 1 µg of RNA
  • Sample type: Peripheral blood
  • Number of reactions per sample: 4
  • Number of targets: 2
  • Manual working time: 45 min
  • Duration of the PCR program: 1h 15 min
  • Compatible cyclers: Real-time PCR cycler with the FAMTM channel


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