Imegen-Quimera Screening Multiplex Plus

Informative molecular marker screening for digital PCR follow-up of allogeneic bone marrow transplant patients

10 rxn (5 transplant cases) -LYOPHILIZED/DEHYDRATED

Real-time PCR

IMG-116-26

7500 FAST Real-Time PCR System (ThermoFisher), StepOnePlus Real-Time PCR System (ThermoFisher), LightCycler 480 (Roche)

  • Multiplexed assays: they allow to determine the presence of 16 polymorphic markers, each one, in recipient and donor samples for the selection of an informative marker for patient follow-up.
  • High sensitivity and specificity: The targets are polymorphic markers of null alleles, insertions or deletions.
  • For the quantification of molecular chimerism, SRY or RhD can also be used if they are positive in the recipient and negative in the donor, knowing the gender and/or blood group of the recipient and the donor.
  • Cumulative global informativeness of the 33 markers together with SRY of 99.98% in the Caucasian population.
  • It complies with the quality requirements specified by ISO 13485 and ISO 14001 in relation to the materials used in its manufacturing process.
  • For in vitro diagnostic use.

Imegen-Quimera Screening Multiplex PlusReal-time multiplexed PCR uses a system of sequence-specific oligonucleotides and hydrolysis probes that allow targets of interest to be specifically amplified.

  • Amount of DNA needed: 450 ng of recipient DNA sample (pre-transplant) and 450 ng of donor DNA sample
  • Sample type: Peripheral blood or bone marrow
  • Number of reactions per recipient sample: 8
  • Number of reactions per donor sample: 8
  • Number of targets: 16
  • Manual working time: 45 min
  • Duration of the PCR program: 1h 20 min
  • Compatible cyclers: real-time PCR cycler with FAMTM and VIC® channels
Reference Kit name Marker
IMG-116-29 Imegen-Quimera Q116-3I dPCR Q116-3I
IMG-116-30 Imegen-Quimera Q116-4I dPCR Q116-4I
IMG-116-31 Imegen-Quimera Q116-5I dPCR Q116-5I
IMG-116-32 Imegen-Quimera Q116-6I dPCR Q116-6I
IMG-116-33 Imegen-Quimera Q116-7I dPCR Q116-7I
IMG-116-34 Imegen-Quimera Q116-11I dPCR Q116-11I
IMG-116-35 Imegen-Quimera Q116-10I dPCR Q116-10I
IMG-116-36 Imegen-Quimera Q116-12D dPCR Q116-12D
IMG-116-37 Imegen-Quimera Q116-23I dPCR Q116-23I
IMG-116-38 Imegen-Quimera Q116-24I dPCR Q116-24I
IMG-116-41 Imegen-Quimera Q116-27D dPCR Q116-27D
IMG-116-42 Imegen-Quimera Q116-28I dPCR Q116-28I
IMG-116-43 Imegen-Quimera Q116-29D dPCR Q116-29D
IMG-116-44 Imegen-Quimera Q116-30D dPCR Q116-30D
IMG-116-45 Imegen-Quimera Q116-31I dPCR Q116-31I
IMG-116-46 Imegen-Quimera Q116-32I dPCR Q116-32I

Imegen-Quimera Screening Multiplex Plus

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