- Multiplexed assays: they allow to determine the presence of 16 polymorphic markers, each one, in recipient and donor samples for the selection of an informative marker for patient follow-up.
- High sensitivity and specificity: The targets are polymorphic markers of null alleles, insertions or deletions.
- For the quantification of molecular chimerism, SRY or RhD can also be used if they are positive in the recipient and negative in the donor, knowing the gender and/or blood group of the recipient and the donor.
- Cumulative global informativeness of the 33 markers together with SRY of 99.98% in the Caucasian population.
- It complies with the quality requirements specified by ISO 13485 and ISO 14001 in relation to the materials used in its manufacturing process.
- For in vitro diagnostic use.
Description
Informative molecular marker screening for digital PCR follow-up of allogeneic bone marrow transplant patients
Format
10 rxn (5 transplant cases) -LYOPHILIZED/DEHYDRATED
Technology
Real-time PCR
Reference
IMG-116-26
Compatible equipment
7500 FAST Real-Time PCR System (ThermoFisher), StepOnePlus Real-Time PCR System (ThermoFisher), LightCycler 480 (Roche)
Real-time multiplexed PCR uses a system of sequence-specific oligonucleotides and hydrolysis probes that allow targets of interest to be specifically amplified.
- Amount of DNA needed: 450 ng of recipient DNA sample (pre-transplant) and 450 ng of donor DNA sample
- Sample type: Peripheral blood or bone marrow
- Number of reactions per recipient sample: 8
- Number of reactions per donor sample: 8
- Number of targets: 16
- Manual working time: 45 min
- Duration of the PCR program: 1h 20 min
- Compatible cyclers: real-time PCR cycler with FAMTM and VIC® channels
| Reference | Kit name | Marker |
| IMG-116-29 | Imegen-Quimera Q116-3I dPCR | Q116-3I |
| IMG-116-30 | Imegen-Quimera Q116-4I dPCR | Q116-4I |
| IMG-116-31 | Imegen-Quimera Q116-5I dPCR | Q116-5I |
| IMG-116-32 | Imegen-Quimera Q116-6I dPCR | Q116-6I |
| IMG-116-33 | Imegen-Quimera Q116-7I dPCR | Q116-7I |
| IMG-116-34 | Imegen-Quimera Q116-11I dPCR | Q116-11I |
| IMG-116-35 | Imegen-Quimera Q116-10I dPCR | Q116-10I |
| IMG-116-36 | Imegen-Quimera Q116-12D dPCR | Q116-12D |
| IMG-116-37 | Imegen-Quimera Q116-23I dPCR | Q116-23I |
| IMG-116-38 | Imegen-Quimera Q116-24I dPCR | Q116-24I |
| IMG-116-41 | Imegen-Quimera Q116-27D dPCR | Q116-27D |
| IMG-116-42 | Imegen-Quimera Q116-28I dPCR | Q116-28I |
| IMG-116-43 | Imegen-Quimera Q116-29D dPCR | Q116-29D |
| IMG-116-44 | Imegen-Quimera Q116-30D dPCR | Q116-30D |
| IMG-116-45 | Imegen-Quimera Q116-31I dPCR | Q116-31I |
| IMG-116-46 | Imegen-Quimera Q116-32I dPCR | Q116-32I |
Imegen-Quimera Screening Multiplex Plus
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