Detection of polymorphisms p.His63Asp, p.Ser65Cys and p.Cys282Tyr in the HFE gene associated with Hemochromatosis

48×3 rxn

Real-time PCR


7500 FAST Real-Time PCR System (ThermoFisher), StepOnePlus Real-Time PCR System (ThermoFisher), StepOne Real-Time PCR System (Thermo Fisher)

  • Multiplex assay that allows determining the presence of normal or pathogenic alleles that give rise to the variants His63Asp (NM_000410.3:c.187C>G), p.Ser65Cys (NM_000410.3:c.193A>T) and p.Cys282Tyr ( NM_000410.3:c.845G>A) in the HFE gene, in a single PCR reaction.
  • It includes a positive control that simulates a heterozygous genotype for each of the polymorphisms, which guarantees the functionality of the PCR system and helps the interpretation in the qualitative analysis.
  • It complies with the quality requirements specified by ISO 13485 and ISO 14001 in relation to the materials used in its manufacturing process.
  • For in vitro diagnostic use.

Imegen-HFE Qualitative real-time PCR assay employing a combination of oligonucleotides and fluorescent hydrolysis probes in a validated assay to simultaneously detect pathogenic p.His63Asp, p.Ser65Cys, and p.Cys282Tyr alleles and normal alleles in the coding region of the HFE gene.

  • Amount of DNA needed: 150 ng total
  • Sample type: Peripheral blood (Germinal)
  • Number of reactions per sample: 3
  • Number of targets: 6
  • Manual working time: 30 min
  • Duration of the PCR program: 1h
  • Compatible cyclers: Real-time PCR cycler with FAMTM and VIC® channels


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