Multiplex assay that allows determining the presence of normal or pathogenic alleles that give rise to the variants His63Asp (NM_000410.3:c.187C>G), p.Ser65Cys (NM_000410.3:c.193A>T) and p.Cys282Tyr ( NM_000410.3:c.845G>A) in the HFE gene, in a single PCR reaction.
It includes a positive control that simulates a heterozygous genotype for each of the polymorphisms, which guarantees the functionality of the PCR system and helps the interpretation in the qualitative analysis.
It complies with the quality requirements specified by ISO 13485 and ISO 14001 in relation to the materials used in its manufacturing process.
For in vitro diagnostic use.
Imegen-HFE Qualitative real-time PCR assay employing a combination of oligonucleotides and fluorescent hydrolysis probes in a validated assay to simultaneously detect pathogenic p.His63Asp, p.Ser65Cys, and p.Cys282Tyr alleles and normal alleles in the coding region of the HFE gene.
Amount of DNA needed: 150 ng total
Sample type: Peripheral blood (Germinal)
Number of reactions per sample: 3
Number of targets: 6
Manual working time: 30 min
Duration of the PCR program: 1h
Compatible cyclers: Real-time PCR cycler with FAMTM and VIC® channels
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