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Redefining
diagnosis
in every read.
Revio™
Unleash the power of HiFi sequencing.
Accuracy, scalability, and simplicity
in your hands.
LONG-READ SEQUENCING
A new paradigm
PacBio long-read sequencing technology generates genomic data through individual reads of 1,000 to 20,000 nucleotides, each derived from a single DNA molecule.
These fragments originate from DNA molecules extracted directly from the biological sample, whereas other high-throughput sequencing technologies require synthetic DNA amplification before analysis.
LONG-READ VS. SHORT-READ
Tell me what you read
and I’ll tell you who you are.
HiFi reads with
accuracy
Long-read technologies, such as PacBio HiFi sequencing, are quickly becoming the new gold standard in genomics research.
With Hi-Fi reads, it is no longer necessary to complement the advantages of long reads with the accuracy of short reads. This technology combines the best of both, allowing for accurate sequencing using long fragments, which is ideal for addressing the most complex biological questions.
HiFi reads
Higher read
quality.
Long-reads
Genomic
assembly with a
higher accuracy.
Not all readings
are fairytales.
| HiFi1,2 | Short-read3 | |
|---|---|---|
| Read length | 1-25 Kb | 50-300 bp |
| Read accuracy | 99,95% | 99,99% |
| Yield | 90 Gb4,5 | Up to 8 Tb |
| Small variants (SNVs + in/dels) |  |
|
| Structural variants (SVs) | |
 |
| Tandem repeats | |
|
| Segment duplications | |
|
| Pseudogenes | |
|
| Methylation | |
|
| Phasing | |
|
(1) Ebert et al. (2021) Science 372(6537):eabf7117
(2) Cohen et al. (2022) Genetic Med doi:10.1016/j.gim.2022.02.007
(3) Byrska-Bishop et al. (2021) bioRxiv doi:10.1101/2021.02.06.430068
(4) The specified HiFi yield is based on HG002/GM24385 human DNA extracted using the Nanobind CBB kit and prepared using the SMRTbell prep 3.0 kit.
(5) HiFi yield depends on library fragment size. Yield is usually lower for shorter libraries.
Revio™.
A giant leap.
PacBio’s Revio platform, thanks to its HiFi technology, expands data yield up to 15-fold compared to previous PacBio platforms and reduces the use of consumables up to 50%.
The platform has four independent sequencing modules that allow for the simultaneous sequencing of four SMRT cells. Each SMRT cell enables the study of one human genome at 30X depth.
Read lengths up to 25 kb and high accuracy (>99.9%) make Revio the ideal option for a wide range of genomic applications, including de novo assembly, comprehensive variant detection, single-cell transcriptomics, and epigenetic characterization.
Uniform coverage.
No bias even for high GC-content regions.
Flexible scalability.
From individual genes to whole genomes.
Longer average
read lengths.
Up to 25-Kb long.
Higher consensus accuracy (HiFi reads).
Minimizes sequencing errors associated with long reads.
Epigenetic characterization.
No need for bisulfite conversion.
Uniform coverage.
Even in hard-to-sequence
or hard-to-align regions.
Haplotype resolution.
It allows differentiating variants in each of the copies of a gene.
It opens the door into a more comprehensive view of biology.
Health in Code is PacBio’s official service provider in southern Europe, supporting small- and large-scale HiFi long-read projects.
How do we work?
MAIN APPLICATIONS
Connecting with the extraordinary.
How to Request a test
To request this test, please follow these steps:
- Click the image to download the form.
- Complete the form with the requested details.
- Send your request to customercare@healthincode.com with the completed form attached in PDF format.